생물 현미경
       실체 현미경
       금속 현미경
       이미지 분석 시스템
       디지털/레이저현미경
 
  HOME/제품소개/생물현미경/ IX/Fluoview/FV 300
  
 
   생물 현미경 (Biolgical Microseope)
  Fluoview     FV 1000    FV 10i


  FLUOVIEW FV1000 Overview  
 

 

 

 

 

 

 

The FV1000 delivers all of the key performance functions required from a confocal laser scanning microscope, minimizes specimen damage during high-speed imaging of living organisms and accurately captures a full range of related information.

Evolved stability: Diode Laser

  • Greater stability, longer service life and lower operating cost are achieved using diode lasers.
  • Reduced heat generation and noise alleviate operator discomfort.
  • With increased power, the same laser can be used for both imaging and laser light stimulation.

Evolved light stimulation: SIM Scanner

  • SIM (SIMultaneous) scanner stimulation synchronized with imaging ensures cell responses during or immediately following stimulation are captured as they happen.
  • The stimulation/imaging positions and laser wavelengths can be set separately with two independent beams.
  • Laser light stimulation position can be changed during imaging.

Evolved performance: High-precision VBF FOR SPECTRAL UNMIXING

High-precision VBF

  • The FV1000 uses a Variable Bandpass Filter (VBF) for maximally utilizing the dispersion of the fluorescence emission to capture freely chosen wavelength ranges.
  • The high precision of the VBF allows acquisition wavelengths to be set freely with 1nm resolution to optimally imagenovel fluorochromes.
  • Separation of fluorescent cross-talk is achieved using the lambda scanning and unmixing functions.

Quantification

  • Laser power monitoring and advanced laser intensity feedback system for stable excitation and stimulation.
  • Diffusion measurement package (DMP), FRET analysis software allow you to get the most from your imaging. Simultaneous stimulation capabilities for the most demanding dynamic experiments.
  • Re-use acquisition parameters for reproducible experiments. Measurement of fluorescent intensity during observation using live-plot.

High Sensitivity

  • Exclusive Analog Accumulation Circuit (AAC) for maximal sensitivity.
  • Uniquely coated filters and dichromatic mirrors enhance sensitivity.
  • Highly sensitive photomultiplier selected specifically for the FV1000.
  • Superior optics for maximal light collection.

 

 
 

Features

Multilaser Combiner

Diode Lasers Encompassing Wide Wavelength Range

The Olympus multi-combiner system can be equipped with diode lasers covering 405nm to 635nm wavelength range. This enables efficient observation of the entire palette of fluorescent proteins. (See spectra below.)

The combination of a 473nm and a 559nm laser is optimal for combinations of EGFP and commonly used red fluorophores. (See below for example images.)

Diode lasers have numerous advantages, including improved stability, reduced heat and noise and reduced operating cost due to low power consumption.

Spectra of commonly used fluorophores.
Spectra of commonly used flurophores

Advantage of 473nm and 559nm diode lasers.
Advantage of 473nm and 559nm diode lasers

UPLSAPO series aberration compensation.

Broadband fiber and UIS2 optics integration for reduced axial chromatic aberration. The system employs infinity corrected (UIS2) objectives, for outstanding optical performance. Color shift and position shift between images is small, ensuring accurate colocalization.